The Homeless Parrot: going to try and explain

Sunday, May 22, 2005

going to try and explain

since amy seems interested, i'm going to try and explain how the cells are made to 'glow' more and also how they are differentiated from one another. bear with me if it gets gory because it's sooooooo neat how they do this. makes me wonder how people come up with these ideas.

ok...so...they take a chicken Thelper cell. all Thelper means is a cell that helps fight off viruses (among other things). and they look at the surface of the T cell and say hey! that T cell has a special little antenna on it called CD4. other T cells (cytotoxic T for instance) and other immune cells don't have that little CD4 antenna. so they take that antenna off the Tcell over years of careful research and money, and they purify it and make a little solution of CD4 antennas. they inject those into a mouse. the mouse's immune system says 'hey! that ain't part of me! it must be bad, let's kill it!' so the mouse mounts an immune response and makes antibody to the CD4 antennas. so now you have something that reacts against the chicken CD4 antenna. that's what an antibody is. so, over many years and with lots of money, the CD4 antibody is isolated from the mouse and stored in little vials with a special fluorescent tag attached and sold for $300 for 0.1mg by southern biotech and the like. i order that and i put it in my chicken blood. and that CD4 antibody recognizes the CD4 antenna sticking out of the Thelper cell and says 'hey! i'm an antibody and i'm supposed to fight bad things! and i recognize that CD4 as a bad thing!' (because it came from the mouse, where CD4 antennas from another species are a bad thing) and it attaches to the CD4 antenna. and voila! now, you can run it through a flow cytometer and you have a little fluorescent tag attached to all your CD4s. that way, you can count how many you have and see if the immune system is working!! it's called mouse anti-CD4 chicken specific monoclonal antibody. (or mAB)

how neat is that?

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Medical terminology

Azotemia - elevation in kidney enzymes (BUN and creatinine) indicating dehydration, kidney failure, or urinary obstruction
Lactate - a salt/ester of lactic acid that is produced as energy for a cell when oxygen levels are low. In critically ill animals, elevated lactate can be an indicator of inadequate blood flow to organs (perfusion), decreased delivery of oxygen, and/or decreased oxygen uptake. Values > 6-7 are usually considered to be poor prognostic indicators for survival.
GI sloughing: when the cells lining the GI tract die (can be secondary to MANY things, including heatstroke) with resulting bloody diarrhea, bacterial translocation into the bloodstream and sepsis
TTJ: transfer to jesus: code for when an animal needs to be euthanized or die
DIC: disseminated intravascular coagulation: a very, very bad thing - when the hemostatic system gets out of whack, and clots start forming in the blood vessels until all clotting factors are wasted. once those are gone, internal hemorrhage ensues, followed by death, usually. also known as "death is coming"
Pleural effusion - fluid contained in the pleural space (chest) - this is not the same as fluid in the lungs (see pulm edema) - in cats can be caused by infection in the chest, heart failure, cancer, FIP, feline leukemia, FIV, and in some cases, the cause is never found (idiopathic)
Anisocoria - unequal pupil size (related to any number of causes including brain damage/head trauma)
Laterally recumbent - lying on side, unable to rise
Hyperglycemia - elevated blood glucose
Hyperkalemia: elevated blood potassium - a life-threatening condition related to several disorders (kidney failure, antifreeze toxicity, urethral obstruction...etc)
Sepsis - refers to a bacterial infection in the bloodstream or body tissues. This is a very broad term covering the presence of many types of microscopic disease-causing organisms.
Nephrectomy - kidney removal
Splenectomy - removal of spleen
Pulmonary edema - condition in which fluid accumulates in the lungs, usually because the heart's left ventricle does not pump adequately ( can be caused by heart failure, electrocution, drowning, too many IV fluids, to name a few)
Tick borne diseases - any of a myriad of diseases transmitted by ticks - including but not limited to Rocky Mtn Spotted fever, Lyme disease, Ehrlichia
Fine needle aspirate - A method of sampling in which a needle is used to suck in cells or tissue bits for diagnoses (good for diagnosing masses/lumps)
Blood glucose - The principal sugar produced by the body from food–especially carbohydrates, but also from proteins and fats; glucose is the body's major source of energy, is transported to cells via the circulation and used by cells in the presence of insulin (normal range in a dog/cat is 75-100)
PCV - packed cell volume - the volume of packed red cells in milliliters per 100 ml of blood (normal range 35-45)

Diseases I see/treat frequently

Dystocia - difficulty birthing. May be responsive to oxytocin administration (Pitocin, as in people) but may require c-section.
DKA - diabetic ketoacidosis: the extreme end of the diabetic scale. A patient that is diabetic can develop DKA when other diseases make the blood glucose hard to regulate. Other diseases that are commonly associated include urinary tract infection, pancreatitis, pyometra, skin infection, and cancer. In DKA, the body starts metabolizing fat and producing acids that cause a drop in blood pH, nausea, weakness, severe dehydration, electrolyte derangments, and death.
DCM - dilated cardiomyopathy: an idiopathic (cause unknown) cardiac disease in which the heart chambers become very thin/dilated, and cardiac output drops radically. Causes arrhythmias, tachycardia, and sudden death. Seen in large breed dogs like Dobermans, Great Danes, etc.
Lymphoma - cancer of the white blood cells, the most common and treatable form of cancer in dogs
Blocked cat - slang term for a male cat with a plug of mucus and crystals obstructing the urethra (fairly common in male cats) definitely a life-threatening because urine can't get out of the body! If present long enough, causes shock, acute renal failure, hyperkalemia (elevated potassium), coma, and death. Symptoms include straining in the litterbox, yowling while trying to urinate, producing small, bloody drops of urine (also symptoms of feline cystitis, a non-lethal condition)
GDV - stands for gastric-dilatation and volvulus - a condition of large breed, deep-chested dogs (usually) in which the stomach rotates 180 degrees on its axis and thus - nothing can enter or leave, considered the "mother of all emergencies" - it warrants immediate surgery and carries a guarded prognosis
IMHA - immune-mediated hemolytic anemia. A disease in which the immune system attacks the red blood cells and destroys them. It causes profound anemia and is life-threatening. Causes are primary (no known cause) and secondary ( tick borne disease, cancer, and heavy metal intoxication). Treatment is immunosuppression with drugs primarily. Prognosis is guarded at best.